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KMID : 0613820170270070805
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Journal of Life Science
2017 Volume.27 No. 7 p.805 ~ p.811
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Characteristics of Hydrolytic Enzymes that Produced by Bacillus subtilis CK-2 Isolated from Doenjang
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Lee Sang-Hyup
Kim Chul-Ho
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Abstract
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In the previous paper, we isolated a bacterium that can hydrolyze various organic materials from soybean paste, including cellulose, lipids, starch, and protein. The activity and chemical properties of the crude enzymes produced by the isolate Bacillus subtilis CK-2 were further investigated. Cellulase showed the highest activity at pH 5.0 and 55℃. The stability of cellulase was maintained within the ranges of pH 5.0~10.0 and 20~50℃. Cellulolytic enzymes were activated by a Co2+ ion, demonstrating the highest activity at a 0.45%(w/v) concentration of Co2+. The optimal conditions for amylase were pH 5.0 and 50℃. The activity of amylase was stable within the ranges of pH 4.0~5.0 and 20~50℃. The Co2+ ion was also necessary for amylase activity, which was the highest at a 0.2%(w/v) concentration of Co2+. The optimal pH and temperature conditions of protease were pH 8.0 and 50℃. The activity of protease was stable within the ranges of pH 7.0~8.5 and 20~50℃. Protease activity was catalyzed by Mn2+, which was the highest at a 0.125%(w/v) concentration of Mn 2+. The isolate B. subtilis CK-2 demonstrated a high activity of autolysin. Based on these results, we identified and suggested the optimal pH, temperature, and metal ion concentration in the use of the hydrolytic enzymes of B. subtilis CK-2 for industrial purposes.
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KEYWORD
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Amylase, Bacillus subtilis, cellulase, optimal condition, protease
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